IKK alpha antibody (pTyr463)

Catalog No. ABIN743273

The Rabbit Polyclonal anti-IKK alpha antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect IKK alpha in samples from Human.

Quick Overview for IKK alpha antibody (pTyr463) (ABIN743273)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IKK alpha Antibody

Binding Specificity: pTyr463

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IKK alpha around the phosphorylation site of Tyr463

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK alpha

Background:

Synonyms: I Kappa B Kinase Alpha, IKKalpha, IKK alpha, IkappaB kinase, IkB kinase alpha subunit, IKBKA, IKK 1, IKK A, IKK a kinase, IKK1, IKKA, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit, NFKBIKA, Nuclear Factor Kappa B Inhibitor Kinase Alpha, Nuclear factor NF kappa B inhibitor kinase alpha, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor, TCF16, CHUK1, Conserved Helix Loop Helix Ubiquitous Kinase, Conserved helix loop ubiquitous kinase, I Kappa B Kinase 1, IKKA_HUMAN.

Background: Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL1, TNF alpha, and bacterial product LPS. NFkB is associated with IkB proteins in the cell cytoplasm, which inhibit NFkB activity. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation. IKK alpha specifically phosphorylates IkBa. IKKa is expressed in variety of human tissues.

Gene ID: 1147

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins