PDX1 antibody (pan)

Catalog No. ABIN7868924

This Rabbit Polyclonal antibody specifically detects PDX1 in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PDX1 antibody (pan) (ABIN7868924)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDX1 Antibody

Binding Specificity: AA 1-246, pan

Purpose: Zebrafish Pdx1 Antibody / Pancreas/duodenum homeobox protein 1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Pdx1 recombinant protein (amino acids M1-S246) was used as the immunogen for the Zebrafish Pdx1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Pdx1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Pdx1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: Pdx1

Background: Pdx1 (Pancreatic and duodenal homeobox 1) is a transcription factor that plays a critical role in pancreatic development and insulin production. It is essential for the development of the pancreas and the maintenance of beta-cell function in adults. Pdx1 regulates the expression of genes involved in pancreatic morphogenesis, beta-cell differentiation, and glucose metabolism.

In zebrafish, Pdx1 is an ortholog of the human PDX1 gene. Both zebrafish and human Pdx1 proteins share significant sequence similarity and functional conservation, particularly in regulating pancreatic development and insulin gene expression. The similarities between the zebrafish and human versions of Pdx1 make zebrafish an excellent model for studying pancreatic biology, beta-cell development, and diabetes.

During embryogenesis, Pdx1 is highly expressed in the developing pancreas, where it directs the differentiation of pancreatic progenitor cells into insulin-producing beta cells. In zebrafish, Pdx1 is essential for the formation of the pancreatic islets and the regulation of insulin secretion in response to blood glucose levels. Pdx1 also plays a key role in beta-cell regeneration after injury, making it a critical factor in pancreatic plasticity.

Zebrafish Pdx1 is expressed predominantly in the pancreas and duodenum during development and in adult stages. The protein regulates the expression of several downstream genes involved in pancreatic endocrine function and beta-cell maturation, including insulin, glucagon, and somatostatin. Pdx1 also interacts with other transcription factors involved in pancreatic development, such as Ngn3 and Pax4, to coordinate the formation of the pancreatic islet.

Given its essential role in pancreatic development, insulin production, and beta-cell regeneration, zebrafish Pdx1 is an important model for studying type 1 and type 2 diabetes, as well as pancreatic diseases and beta-cell dysfunction. The zebrafish model offers a unique opportunity to study pancreatic regeneration, gene regulation, and metabolic disorders in real time.

UniProt: Q6DC85

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location