PPARG antibody (AA 1-512)

Catalog No. ABIN7869446

This Rabbit Polyclonal antibody specifically detects PPARG in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PPARG antibody (AA 1-512) (ABIN7869446)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPARG Antibody

Binding Specificity: AA 1-512

Purpose: Zebrafish Ppar gamma Antibody / Pparg

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Ppar gamma recombinant protein (amino acids M1-D512) was used as the immunogen for the Zebrafish Ppar gamma antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Ppar gamma antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Ppar gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: Ppar gamma

Background: Pparg (Peroxisome proliferator-activated receptor gamma) is a critical transcription factor that belongs to the nuclear receptor superfamily. It plays a vital role in regulating lipid metabolism, glucose homeostasis, inflammation, and adipocyte differentiation. As a ligand-activated transcription factor, Pparg regulates the expression of genes involved in a variety of physiological processes, including energy metabolism, insulin sensitivity, and immune response. Pparg is activated by fatty acids and other lipid metabolites, making it a key regulator of metabolic homeostasis.

In zebrafish, Pparg is an ortholog of the human PPARG gene. The zebrafish and human proteins share a high degree of sequence conservation, particularly in their DNA binding domain and ligand-binding domain, which are essential for their function as transcription factors. This evolutionary conservation suggests that the role of Pparg in regulating lipid metabolism and adipogenesis is preserved across species, making zebrafish a valuable model for studying the molecular mechanisms of metabolic diseases and obesity.

Pparg in zebrafish has isoforms, which may exhibit tissue-specific expression and functional variation. The existence of isoforms allows for regulation of different target genes depending on developmental stages, tissue types, and environmental conditions. These isoforms may also be involved in regulating distinct physiological processes, such as fatty acid metabolism, inflammatory response, and immune cell differentiation.

In zebrafish, Pparg is expressed in various tissues, including the adipose tissue, liver, and muscles, as well as in developing embryos. Its expression is tightly regulated to ensure proper energy homeostasis and organ development. Pparg plays an essential role in early development, especially in regulating the formation of adipocytes (fat cells), which are important for long-term energy storage and regulation. Disruption of Pparg function in zebrafish can result in defects in lipid metabolism and adipogenesis, providing a model for studying metabolic disorders.

UniProt: A6XMH6

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation