HK3 antibody (AA 1-789)

Catalog No. ABIN7869557

This Rabbit Polyclonal antibody specifically detects HK3 in WB, ELISA, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for HK3 antibody (AA 1-789) (ABIN7869557)

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-HK3 Antibody

Binding Specificity: AA 1-789

Purpose: Hexokinase 3 Antibody / HK3

Purification: Antigen affinity purified

Immunogen: A human recombinant partial protein (amino acids M1-K789) was used as the immunogen for the Hexokinase 3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Hexokinase 3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Hexokinase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HK3

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Alternative Name: Hexokinase 3

Background: Hexokinase 3 (HK3) is a member of the hexokinase enzyme family, which catalyzes the first committed step in glucose metabolism. Like other isoforms, HK3 phosphorylates glucose to produce glucose-6-phosphate, a critical intermediate in glycolysis and the pentose phosphate pathway. This phosphorylation step traps glucose inside the cell and directs it into energy-producing and biosynthetic pathways. Researchers often use a Hexokinase 3 antibody to study cellular metabolism, energy regulation, and metabolic diseases.

HK3 differs from other hexokinase isoforms in its tissue distribution and regulatory properties. It is predominantly expressed in leukocytes and other hematopoietic cells, suggesting a specialized role in immune cell metabolism. Unlike HK1 and HK2, HK3 does not strongly associate with the outer mitochondrial membrane, which may influence its role in apoptosis and energy regulation. Employing a Hexokinase 3 antibody allows scientists to explore its unique expression patterns and its contributions to both glycolysis and immune cell biology.

Altered expression of HK3 has been observed in cancer and inflammatory conditions, where shifts in glucose metabolism play a role in disease progression. HK3 has also been implicated in oxidative stress responses and metabolic reprogramming of immune cells. As interest in immunometabolism continues to grow, HK3 remains an important protein for research into metabolic regulation and disease. Using a Hexokinase 3 antibody enables reliable detection of this isoform in various biological contexts.

NSJ Bioreagents provides a high-quality Hexokinase 3 antibody validated for applications such as western blot, immunohistochemistry, and immunofluorescence. Choosing a Hexokinase 3 antibody from NSJ Bioreagents ensures consistent performance and reproducible results in studies of glucose metabolism, immunometabolism, and disease mechanisms.

UniProt: P52790

Pathways: Carbohydrate Homeostasis, Warburg Effect