FBXW7 antibody (AA 151-591)

Catalog No. ABIN7870975

The Rabbit Polyclonal anti-FBXW7 antibody (ABIN7870975) specifically detects FBXW7 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FBXW7 antibody (AA 151-591) (ABIN7870975)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-FBXW7 Antibody

Binding Specificity: AA 151-591

Purpose: FBXW7 Antibody / F-box/WD repeat-containing protein 7

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human FBXW7 recombinant protein (Position: D151-D591) was used as the immunogen for the FBXW7 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FBXW7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FBXW7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7

Background: FBXW7 antibody detects F-box/WD repeat-containing protein 7, encoded by the FBXW7 gene on chromosome 4q31.1. FBXW7 antibody is widely used to investigate this tumor suppressor protein, which functions as a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex. FBXW7 plays a central role in regulating proteasomal degradation of key cell cycle regulators and oncogenic proteins. By controlling turnover of cyclin E, c-Myc, Notch, mTOR, and Jun, FBXW7 maintains cell cycle integrity, suppresses uncontrolled proliferation, and modulates signaling pathways fundamental to development and tumorigenesis.

Structurally, FBXW7 contains three conserved domains: an F-box motif that mediates interaction with SKP1, WD40 repeats that form a propeller-like structure for substrate recognition, and dimerization regions that enhance binding affinity. The WD40 domain binds phosphorylated degrons in substrates, ensuring phosphorylation-dependent degradation. FBXW7 recognizes multiple oncoproteins via these motifs, linking post-translational modifications to proteasomal clearance. Isoforms generated by alternative splicing-alpha, beta, and gamma-differ in subcellular localization and tissue expression, broadening its regulatory scope.

FBXW7's tumor suppressor function is underscored by frequent mutations in cancers. Loss-of-function mutations destabilize the SCF complex and permit accumulation of oncogenic proteins, driving tumor progression. FBXW7 mutations are prevalent in colorectal, gastric, breast, endometrial, and T-cell acute lymphoblastic leukemias. Its inactivation contributes to chemoresistance, genomic instability, and poor prognosis. Conversely, intact FBXW7 promotes cell cycle arrest, apoptosis, and maintenance of stem cell homeostasis. Researchers rely on FBXW7 antibody to evaluate its expression, detect isoforms, and monitor degradation pathways of oncogenic substrates.

FBXW7 is also critical in developmental biology. It regulates Notch signaling during neural development, vascular morphogenesis, and stem cell differentiation. Disruption of FBXW7 in animal models results in embryonic lethality or tissue-specific developmental defects. Beyond cancer, FBXW7 contributes to neurological disorders and metabolic diseases by modulating protein degradation pathways. Its regulatory roles in mTOR signaling tie it to metabolism, autophagy, and aging-related processes.

Experimentally, FBXW7 antibody is applied in western blotting to detect isoform-specific expression, in immunohistochemistry to evaluate tumor samples, and in immunoprecipitation to analyze SCF complex formation. Studies using FBXW7 antibody confirm interactions with GSK3b-phosphorylated degrons and highlight dynamic regulation of substrates across cell cycle phases. NSJ Bioreagents provides FBXW7 antibody for cancer biology, proteostasis research, and translational studies exploring ubiquitin ligase function.

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway