PFKM antibody (AA 39-780)

Catalog No. ABIN7874608

The Rabbit Polyclonal anti-PFKM antibody (ABIN7874608) specifically detects PFKM in WB, IHC, ELISA, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PFKM antibody (AA 39-780) (ABIN7874608)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PFKM Antibody

Binding Specificity: AA 39-780

Purpose: PFKM Antibody / Phosphofructokinase muscle type

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PFKM recombinant protein (Position: R39-V780) was used as the immunogen for the PFKM antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PFKM antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PFKM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM

Background: PFKM antibody detects Phosphofructokinase, muscle type, a key glycolytic enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. The UniProt recommended name is ATP-dependent 6-phosphofructokinase, muscle type (PFKM). This enzyme controls a rate-limiting step in glycolysis and is a central regulator of glucose metabolism and energy production in muscle tissue.

Functionally, PFKM antibody identifies a 780-amino-acid cytosolic enzyme that forms homo- or heterotetramers with other phosphofructokinase isoforms (PFKL and PFKP). PFKM activity is allosterically regulated by ATP, citrate, AMP, and fructose-2,6-bisphosphate, allowing fine-tuned control of glycolytic flux according to cellular energy status. Its catalytic action commits glucose to the glycolytic pathway, linking energy demand with carbohydrate metabolism.

The PFKM gene is located on chromosome 12q13.11 and is predominantly expressed in skeletal and cardiac muscle. It plays a vital role in maintaining muscle energy supply during contraction. PFKM expression and activity adapt to metabolic cues, hypoxia, and hormonal regulation, integrating glycolysis with systemic energy homeostasis.

Pathologically, mutations in PFKM cause Glycogen storage disease type VII (Tarui disease), characterized by exercise intolerance, muscle cramps, and myoglobinuria. Deficiency leads to impaired glycolytic ATP production and accumulation of glycogen in muscle fibers. Aberrant PFKM regulation is also associated with metabolic disorders and tumor glycolysis (Warburg effect). Research with PFKM antibody supports studies in metabolism, enzymology, and inherited myopathies.

PFKM antibody is suitable for western blotting, immunohistochemistry, and enzyme assays to detect muscle phosphofructokinase expression. NSJ Bioreagents provides validated PFKM antibody reagents optimized for glycolysis, muscle physiology, and metabolic disease research.

Structurally, Phosphofructokinase, muscle type forms a tetrameric enzyme with alternating catalytic and regulatory domains. Allosteric binding sites modulate its conformational state and enzymatic efficiency. This antibody allows detailed analysis of PFKM's contribution to energy metabolism and metabolic control.

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect