RASAL3 antibody (AA 48-925)

Catalog No. ABIN7875219

The Rabbit Polyclonal anti-RASAL3 antibody is suitable to detect RASAL3 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for RASAL3 antibody (AA 48-925) (ABIN7875219)

Target: RASAL3 (RAS Protein Activator Like 3 (RASAL3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASAL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-RASAL3 Antibody

Binding Specificity: AA 48-925

Purpose: RASAL3 Antibody / Ras GTPase-activating-like protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RASAL3 recombinant protein (Position: R48-Q925) was used as the immunogen for the RASAL3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RASAL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RASAL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RASAL3

Target: RASAL3 (RAS Protein Activator Like 3 (RASAL3))

Alternative Name: RASAL3

Background: RASAL3 antibody detects Ras GTPase-activating-like protein 3, a Ras signaling regulator that acts as a negative modulator of Ras activity in immune cells. The UniProt recommended name is Ras GTPase-activating-like protein 3 (RASAL3). This protein converts active GTP-bound Ras into its inactive GDP-bound form, thereby controlling proliferation and activation signals in lymphocytes and other hematopoietic cells.

Functionally, RASAL3 antibody identifies a 944-amino-acid cytoplasmic protein containing C2 and RasGAP domains responsible for lipid membrane binding and GTPase activation. RASAL3 inhibits Ras-dependent pathways, including ERK/MAPK signaling, limiting excessive immune cell activation and promoting homeostasis. It acts downstream of receptor stimulation to fine-tune immune responses.

The RASAL3 gene is located on chromosome 19q13.3 and is highly expressed in natural killer cells, T cells, and macrophages. It serves as a negative feedback regulator of immune activation by controlling Ras signaling intensity following antigen or cytokine stimulation. In NK cells, RASAL3 influences cytotoxic granule release and antiviral responses.

Pathologically, dysregulation of RASAL3 contributes to immune dysfunction and inflammation. Loss of function enhances Ras activity, promoting lymphoproliferative and autoimmune phenotypes. In cancer, RASAL3 downregulation may facilitate tumor immune evasion by disturbing immune regulation. Research using RASAL3 antibody supports investigations into Ras pathway modulation, immune signaling, and oncogenic Ras control.

RASAL3 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect RasGAP proteins and Ras activity regulators. NSJ Bioreagents offers RASAL3 antibody reagents optimized for studies in immune signaling, GTPase biology, and cellular regulation.

Structurally, Ras GTPase-activating-like protein 3 contains an N-terminal C2 lipid-binding domain and a C-terminal catalytic GAP domain. This architecture enables membrane association and inactivation of Ras. This antibody facilitates research on RASAL3's inhibitory role in signal transduction and immune modulation.

UniProt: Q86YV0