Glutaminase antibody (AA 516-545)

Catalog No. ABIN7875535

The Rabbit Polyclonal anti-Glutaminase antibody (ABIN7875535) specifically detects Glutaminase in WB, IF, FACS and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Glutaminase antibody (AA 516-545) (ABIN7875535)

Target: Glutaminase (GLS)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Glutaminase Antibody

Binding Specificity: AA 516-545

Purpose: Glutaminase Antibody / GLS

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 516-545 from the human protein were used as the immunogen for the Glutaminase antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Glutaminase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Glutaminase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: Glutaminase

Background: Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect