ROCK1 antibody (AA 601-1319)

Catalog No. ABIN7876062

The Rabbit Polyclonal anti-ROCK1 antibody is suitable to detect ROCK1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for ROCK1 antibody (AA 601-1319) (ABIN7876062)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ROCK1 Antibody

Binding Specificity: AA 601-1319

Purpose: ROCK1 Antibody / Rho-associated protein kinase 1

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids K601-N1319) was used as the immunogen for the ROCK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ROCK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ROCK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: ROCK1 antibody targets Rho-associated protein kinase 1, encoded by the ROCK1 gene. Rho-associated protein kinase 1 is a serine-threonine kinase that functions as a key downstream effector of the small GTPase RhoA. ROCK1 belongs to the AGC family of protein kinases and plays a central role in regulating the actin cytoskeleton, cell shape, and contractile force generation through phosphorylation of multiple cytoskeletal and signaling substrates.

Functionally, Rho-associated protein kinase 1 mediates actomyosin contractility by phosphorylating targets such as myosin light chain and myosin phosphatase subunits, thereby promoting stress fiber formation and focal adhesion assembly. ROCK1 signaling influences diverse cellular processes including cell migration, adhesion, cytokinesis, and apoptosis. A ROCK1 antibody supports studies focused on Rho GTPase signaling pathways, cytoskeletal dynamics, and mechanical regulation of cell behavior.

ROCK1 is broadly expressed across many tissues and cell types, reflecting its fundamental role in cellular architecture and signaling. Subcellular localization is predominantly cytoplasmic, with enrichment at actin filaments, stress fibers, and contractile structures, although localization can change in response to RhoA activation and cellular context. During apoptotic signaling, ROCK1 can undergo proteolytic cleavage that alters its activity and functional output, further highlighting its regulatory versatility.

From a disease relevance perspective, dysregulated ROCK1 activity has been investigated in cancer, cardiovascular disease, fibrosis, and neurological disorders, where aberrant cytoskeletal remodeling and cell motility contribute to disease progression. At the molecular level, Rho-associated protein kinase 1 contains an N-terminal kinase domain, a central coiled-coil region with a Rho-binding domain, and C-terminal regulatory motifs that control autoinhibition and activation. ROCK1 antibody reagents support research applications examining cytoskeletal signaling, cell contractility, and Rho-mediated pathways, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling