PLK1 antibody (full length)

Catalog No. ABIN7878824

This Mouse Monoclonal antibody specifically detects PLK1 in WB and ELISA. It exhibits reactivity toward Xenopus laevis.

Quick Overview for PLK1 antibody (full length) (ABIN7878824)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: AZ44

Product Details anti-PLK1 Antibody

Binding Specificity: full length

Purpose: PLK1 Antibody / Polo-Like Kinase 1

Purification: Protein G affinity chromatography

Immunogen: Recombinant Xenopus laevis full length protein was used as the immunogen for the PLK1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the PLK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PLK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle, Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase.

UniProt: P70032

Pathways: Cell Division Cycle, M Phase