PAG1 antibody (N-Term)

Catalog No. ABIN7879646

This Rabbit Polyclonal antibody specifically detects PAG1 in WB, FACS, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAG1 antibody (N-Term) (ABIN7879646)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAG1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PAG1 Antibody

Binding Specificity: N-Term

Purpose: PAG1 Antibody / Cbp / Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PAG1 was used as the immunogen for the PAG1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PAG1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PAG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Alternative Name: PAG1

Background: PAG1 antibody detects Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, also called Transmembrane phosphoprotein Cbp, a transmembrane adaptor protein that regulates Src family kinase signaling and immune receptor function. The UniProt recommended name is Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1). This scaffold protein, also known as Csk-binding protein (CBP), localizes to lipid rafts in the plasma membrane and mediates inhibitory signaling by recruiting the tyrosine kinase CSK to suppress Src family kinases such as LCK and FYN.

Functionally, PAG1 antibody identifies a 432-amino-acid type I transmembrane protein containing multiple tyrosine residues that undergo phosphorylation following receptor activation. Phosphorylated PAG1 binds CSK, leading to phosphorylation and inactivation of Src kinases at their C-terminal regulatory tyrosine. This mechanism provides a critical negative feedback loop that fine-tunes T cell receptor (TCR) and B cell receptor (BCR) signaling, maintaining immune homeostasis.

The PAG1 gene is located on chromosome 8q21.13 and is expressed in hematopoietic cells, neurons, and various epithelial tissues. In T lymphocytes, PAG1 is enriched in membrane microdomains and associates with LAT, LCK, and CD45, modulating activation thresholds and signaling kinetics. Beyond the immune system, PAG1 participates in neuronal development, cell adhesion, and stress response signaling pathways.

Pathologically, dysregulated PAG1 expression contributes to altered immune signaling and oncogenic kinase activation. Downregulation of PAG1 has been observed in certain lymphomas and leukemias, allowing unchecked Src kinase activity. Conversely, overexpression may suppress cell motility and proliferation. Research using PAG1 antibody supports investigations into lipid raft-associated signaling, immune synapse organization, and signal attenuation mechanisms.

PAG1 antibody is suitable for use in western blotting, immunoprecipitation, and immunofluorescence to detect native or phosphorylated PAG1 in cell lysates or tissue samples. It supports studies focused on immune receptor regulation, kinase signaling networks, and cancer biology. NSJ Bioreagents offers validated PAG1 antibody reagents designed for reliable performance in Src signaling and membrane microdomain research.

Structurally, PAG1 contains an extracellular immunoglobulin-like domain, a single transmembrane segment, and a cytoplasmic tail rich in tyrosine motifs. Its lipid raft localization depends on palmitoylation and interaction with glycosphingolipids. This antibody aids in elucidating PAG1's scaffolding functions that link plasma membrane signaling to intracellular kinase regulation.

UniProt: Q9NWQ8

Pathways: p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events