Recombinant RPS6KB2 antibody (pSer371)

Catalog No. ABIN7879759

The Rabbit Monoclonal anti-RPS6KB2 antibody (Clone 32R04) (ABIN7879759) specifically detects RPS6KB2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant RPS6KB2 antibody (pSer371) (ABIN7879759)

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPS6KB2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32R04

Product Details anti-RPS6KB2 Antibody

Binding Specificity: pSer371

Purpose: Phospho-p70 S6 Kinase beta (Ser371) Antibody

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-P70 S6 Kinase beta (S371) was used as the immunogen for the Phospho-p70 S6 Kinase beta (Ser371) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-p70 S6 Kinase beta (Ser371) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-p70 S6 Kinase beta (Ser371) antibody at -20oC.

Target Details for RPS6KB2

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Alternative Name: p70 S6 Kinase beta

Background: Phospho-p70 S6 Kinase beta (Ser371) antibody detects the phosphorylated form of ribosomal protein S6 kinase beta-1 (S6K1) at serine 371. S6K1, also called p70S6K, is a serine/threonine kinase activated downstream of mTORC1 and plays a pivotal role in protein synthesis, cell growth, and metabolism. Phosphorylation at Ser371 is an essential priming event for full kinase activation, enabling subsequent phosphorylation at the hydrophobic motif and activation loop.

Research using Phospho-p70 S6 Kinase beta (Ser371) antibody highlights the importance of this modification in cell growth and proliferation. Ser371 phosphorylation enhances kinase activity, promoting phosphorylation of ribosomal protein S6 and translation initiation factors. This activity stimulates protein synthesis, supporting anabolic metabolism and cellular hypertrophy. In cancer, aberrant activation of S6K1 contributes to uncontrolled growth and resistance to apoptosis. Elevated Ser371 phosphorylation has been observed in breast, prostate, and colorectal tumors, where it correlates with mTORC1 hyperactivation.

Phosphorylation at Ser371 is also a readout of nutrient and growth factor signaling. Under nutrient-rich conditions, mTORC1 activity drives S6K1 phosphorylation, whereas nutrient deprivation or rapamycin treatment diminishes this event. Monitoring Ser371 phosphorylation provides insight into mTOR pathway regulation in metabolic disease and therapeutic interventions. In insulin resistance and type 2 diabetes, dysregulated S6K1 signaling contributes to feedback inhibition of insulin receptor substrates, linking it to impaired glucose homeostasis.

Neurobiology studies show that S6K1 activity influences synaptic plasticity, memory, and neuronal survival. Ser371 phosphorylation may integrate mTOR activity with cognitive functions, while dysregulation is implicated in neurodegenerative disorders. In cardiovascular research, hyperactivation of S6K1 contributes to pathological hypertrophy and vascular remodeling.

Antibodies against phospho-S6K1 (Ser371) are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents specifically recognize the phosphorylated form, enabling distinction between active and inactive states. Clone-based antibodies provide reproducibility for studying signaling pathways, drug responses, and disease mechanisms.

UniProt: Q9UBS0

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway