Recombinant Smooth Muscle Actin antibody

Catalog No. ABIN7879878

The Rabbit Monoclonal anti-Smooth Muscle Actin antibody is suitable to detect Smooth Muscle Actin in samples from Human, Rat and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for Recombinant Smooth Muscle Actin antibody (ABIN7879878)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EO-1

Product Details anti-Smooth Muscle Actin Antibody

Purpose: ACTA2 Antibody / Alpha Actin / Smooth Muscle Actin

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human Smooth muscle actin was used as the immunogen for the ACTA2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ACTA2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the ACTA2 antibody at -20oC.

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Background: ACTA2 antibody is a widely used reagent for studying cytoskeletal organization, smooth muscle biology, and tissue remodeling. The encoded protein, alpha actin (ACTA2), is an isoform of actin expressed primarily in vascular and visceral smooth muscle. As a major component of the contractile apparatus, ACTA2 plays a central role in generating force for smooth muscle contraction and maintaining structural integrity of the cytoskeleton.

Alpha actin is abundantly expressed in smooth muscle cells of the vasculature, airways, gastrointestinal tract, and reproductive system. It is also used as a marker of myofibroblast differentiation, where its upregulation reflects tissue remodeling during wound healing, fibrosis, and pathological scarring. Because of its strong and specific expression pattern, detection with ACTA2 antibody is widely applied in immunohistochemistry and immunofluorescence to identify smooth muscle or myofibroblastic cells in tissue samples.

In cardiovascular research, ACTA2 is studied as a marker of vascular smooth muscle cells and their phenotypic switching. Under pathological conditions such as atherosclerosis or hypertension, ACTA2 expression can change as smooth muscle cells adopt synthetic phenotypes. In pulmonary and hepatic fibrosis, ACTA2 positive myofibroblasts contribute to extracellular matrix deposition and tissue stiffening, making ACTA2 a reliable indicator of fibrotic remodeling.

Structurally, alpha actin is a globular protein that polymerizes into filamentous actin, forming part of the cytoskeleton. ACTA2 interacts with actin binding proteins to regulate filament dynamics, cell shape, and motility. Its high conservation across species ensures that ACTA2 antibody is effective in many experimental systems, including human and animal models.

The ACTA2 antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. These applications enable researchers to assess expression, distribution, and structural changes of smooth muscle actin in both healthy and diseased tissues. For scientists investigating vascular biology, fibrosis, tissue remodeling, or smooth muscle physiology, the ACTA2 antibody provides a sensitive and specific detection tool. NSJ Bioreagents offers validated antibodies that ensure reproducibility and accuracy in advanced molecular studies.

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development