Recombinant PPARG antibody

Catalog No. ABIN7881920

The Rabbit Monoclonal anti-PPARG antibody (Clone 31P97) (ABIN7881920) specifically detects PPARG in WB, ICC, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Recombinant PPARG antibody (ABIN7881920)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 31P97

Product Details anti-PPARG Antibody

Purpose: PPAR gamma Antibody / PPARG

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human PPAR gamma was used as the immunogen for the PPAR gamma antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PPAR gamma antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PPAR gamma antibody at -20oC.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPAR gamma

Background: PPAR gamma antibody targets peroxisome proliferator activated receptor gamma, a nuclear receptor encoded by the PPARG gene. PPAR gamma functions as a ligand activated transcription factor and plays essential roles in adipocyte differentiation, glucose metabolism, and lipid storage. It binds to specific DNA sequences as a heterodimer with RXR, regulating genes involved in insulin sensitivity, fatty acid uptake, and inflammatory responses.

PPAR gamma exists in at least two isoforms, PPAR gamma 1 and PPAR gamma 2, which differ in tissue distribution and regulatory control. PPAR gamma 1 is broadly expressed, while PPAR gamma 2 is more adipocyte specific. Both isoforms are activated by endogenous ligands such as prostaglandin derivatives and polyunsaturated fatty acids, as well as synthetic ligands like thiazolidinediones. These synthetic agonists have been widely used as antidiabetic medications, making PPAR gamma antibody an important reagent in metabolic disease research.

The receptor is also implicated in cancer biology. PPAR gamma can regulate cell cycle arrest, apoptosis, and differentiation. Studies using PPAR gamma antibody have shown altered expression in breast, colon, and prostate cancers. Inflammation research has further demonstrated that PPAR gamma modulates immune responses by suppressing pro inflammatory cytokine production in macrophages and T cells.

Detection of PPAR gamma with PPAR gamma antibody is routinely carried out in western blotting, immunohistochemistry, and chromatin immunoprecipitation. Western blotting reveals isoform specific expression, while immunohistochemistry highlights nuclear localization. Chromatin immunoprecipitation assays combined with next generation sequencing have uncovered PPAR gamma binding sites across the genome, providing insights into transcriptional networks controlling energy homeostasis.

PPAR gamma remains a therapeutic target for type 2 diabetes, obesity, and cardiovascular disease. Researchers rely on PPAR gamma antibody to study receptor expression, ligand responsiveness, and downstream signaling changes under physiological and pathological conditions. Availability of validated antibodies such as those from NSJ Bioreagents supports reproducible discovery in metabolic research and drug development.

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation