Recombinant ROCK1 antibody

Catalog No. ABIN7882164

This Rabbit Monoclonal antibody specifically detects ROCK1 in WB, IHC, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant ROCK1 antibody (ABIN7882164)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 32R84

Product Details anti-ROCK1 Antibody

Purpose: ROCK1 Antibody / Rho associated coiled coil containing protein kinase 1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human ROCK1 was used as the immunogen for the ROCK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ROCK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the ROCK1 antibody at -20oC.

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: ROCK1 antibody detects Rho associated coiled coil containing protein kinase 1, encoded by the ROCK1 gene. Rho associated coiled coil containing protein kinase 1 is a serine-threonine kinase that mediates actin cytoskeleton organization, cell adhesion, and motility downstream of Rho GTPases. ROCK1 antibody provides researchers with an important reagent to study signal transduction, cytoskeletal remodeling, and disease processes.

Rho associated coiled coil containing protein kinase 1 is activated by binding to the small GTPase RhoA. Research using ROCK1 antibody has shown that upon activation, the kinase phosphorylates substrates such as LIM kinase, myosin light chain phosphatase, and ERM proteins. These phosphorylation events regulate actin filament assembly, stress fiber formation, and focal adhesion dynamics. Through these activities, ROCK1 coordinates changes in cell shape and migration in response to extracellular signals.

Studies with ROCK1 antibody have revealed that the kinase is essential for smooth muscle contraction, axon guidance, and angiogenesis. ROCK1 participates in vascular tone regulation by modulating smooth muscle contractility, while in the nervous system, it influences growth cone dynamics during axonal pathfinding. In endothelial cells, ROCK1 activity supports sprouting angiogenesis and vessel stability. These diverse roles emphasize its broad physiological importance.

Dysregulation of Rho associated coiled coil containing protein kinase 1 contributes to disease. Research using ROCK1 antibody has demonstrated that aberrant activation promotes cancer cell invasion and metastasis by remodeling the actin cytoskeleton. In cardiovascular disease, excessive ROCK1 activity contributes to vascular stiffness and hypertension. In neurological disorders, altered ROCK1 function affects axonal regeneration and contributes to neurodegeneration. These findings highlight ROCK1 as a therapeutic target in multiple pathological contexts.

Pharmacological inhibition of ROCK1 has been explored for therapeutic benefit. Studies with ROCK1 antibody have supported the development of selective inhibitors such as fasudil and ripasudil, which show efficacy in treating pulmonary hypertension, glaucoma, and other conditions involving abnormal ROCK signaling. This underscores the clinical relevance of monitoring ROCK1 expression and activity.

ROCK1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects kinase expression and post-translational modifications, immunohistochemistry demonstrates tissue distribution, and immunofluorescence shows cytoplasmic localization associated with actin fibers. These applications make ROCK1 antibody versatile in cell biology and disease research.

By providing validated ROCK1 antibody reagents, NSJ Bioreagents supports research into cytoskeletal regulation, signaling, and therapeutic development. Detection of Rho associated coiled coil containing protein kinase 1 provides insight into how Rho GTPase pathways govern cellular architecture and disease mechanisms.

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling