Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 1039-1054), (Intracellular) antibody

Catalog No. ABIN7885365

This Rabbit Polyclonal antibody specifically detects Regulating Synaptic Membrane Exocytosis 1 in WB. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 1039-1054), (Intracellular) antibody (ABIN7885365)

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details

Binding Specificity: AA 1039-1054, Intracellular

Purpose: A Rabbit Polyclonal Antibody to RIM1

Predicted Reactivity: human - 14,16 amino acid residues identical,Mouse - 15

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236714)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Alternative Name: Regulating synaptic membrane exocytosis 1

Background:

Synonyms: Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1

Description: RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β, RIM2α, 2β, 2γ, and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

Gene ID: 84556

UniProt: Q9JIR4

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity