Serotonin Receptor 4 antibody (2nd Extracellular Loop)

Catalog No. ABIN7885394

The Rabbit Polyclonal anti-Serotonin Receptor 4 antibody (ABIN7885394) specifically detects Serotonin Receptor 4 in WB, FACS, LCI and IC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Serotonin Receptor 4 antibody (2nd Extracellular Loop) (ABIN7885394)

Target: Serotonin Receptor 4 (HTR4)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Grade: Carrier-free, KO Validated

Product Details anti-Serotonin Receptor 4 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 168-180

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4

Predicted Reactivity: Mouse - 12,13 amino acid residues identical, human - 11

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Alternative Name: 5-Hydroxytryptamine receptor 4

Background:

Synonyms: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Description: 5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Gene ID: 25324

UniProt: Q62758

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins