SYN1 antibody (C Region, Intracellular)

Catalog No. ABIN7885547

The Rabbit Polyclonal anti-SYN1 antibody (ABIN7885547) specifically detects SYN1 in WB, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SYN1 antibody (C Region, Intracellular) (ABIN7885547)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-SYN1 Antibody

Binding Specificity: AA 234-245, C Region, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Synapsin I

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RLHKKLGTEEFP(G), corresponding to amino acid residues 234 - 245 of rat Synapsin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237307)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin-1

Background:

Synonyms: Synapsin-1

Description: Synapsins are a family of synaptic vesicle associated phosphoproteins consisting of three members: SYN1, SYN2, and SYN3, with several alternative splicing leading to 10 different isoforms. They are involved in synaptic transmission and plasticity, several stages of neurodevelopment, such as axon outgrowth and synapse formation. Synapsins are localized in presynaptic nerve terminals of central neurons1.Synapsin I is a peripheral membrane protein of synaptic vesicles that is phosphorylated at least at three different sites by various protein kinases. All synapsins have a single phosphorylation site in the short N-terminal domain A (site 1) that regulates neurite elongation and synaptic vesicle mobilization2.Synapsin I exhibits an asymmetric structure and exists in two forms, designated synapsin Ia and synapsin Ib. The primary structure of synapsin I is highly conserved, 95 % of the protein's residues are invariant and contain two major structural domains. The protein is divided into a hydrophobic, collagenase resistant head region and a proline rich, collagenase sensitive tail3,4. Synapsin I has been suggested to play an important role in the regulation of neurotransmitter release and the organization of cytoskeletal organization including actin filaments, microtubules and spectrin in the presynaptic terminal5.In vitro studies show that the dephospho-form of synapsin I binds synaptic vesicles, binds and bundles F-actin, and promotes G actin polymerization5.

Gene ID: 24949

UniProt: P09951