Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ARAF antibody (pSer299) (FITC)

This anti-ARAF antibody is a Rabbit Polyclonal antibody detecting ARAF in WB, IF (cc) and IF (p). Suitable for Human.
Catalog No. ABIN745335

Quick Overview for ARAF antibody (pSer299) (FITC) (ABIN745335)

Target

See all ARAF Antibodies
ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity

  • 108
  • 40
  • 21
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
Human

Host

  • 103
  • 5
Rabbit

Clonality

  • 94
  • 14
Polyclonal

Conjugate

  • 51
  • 6
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ARAF antibody is conjugated to FITC

Application

  • 73
  • 41
  • 39
  • 38
  • 20
  • 18
  • 13
  • 9
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • Binding Specificity

    • 19
    • 17
    • 16
    • 15
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer299

    Cross-Reactivity

    Human

    Predicted Reactivity

    Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser299

    Isotype

    IgG
  • Application Notes

    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

    Alternative Name

    A Raf

    Background

    Synonyms: A RAF phospho S299, p-A RAF phospho S299,A raf 1, A Raf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, ARaf proto oncogene serine/threonine protein kinase, ARAF1, Oncogene Araf1, Oncogene PKS2, PKS 2, PKS, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, ARAF_HUMAN.

    Background: The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.

    Gene ID

    369

    UniProt

    P10398

    Pathways

    MAPK Signaling, RTK Signaling, Hepatitis C
You are here:
Chat with us!