ARAF antibody (pSer299) (FITC)

Catalog No. ABIN745335

The FITC-conjugated Rabbit Polyclonal anti-ARAF antibody (ABIN745335) specifically detects ARAF in WB, IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for ARAF antibody (pSer299) (FITC) (ABIN745335)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ARAF Antibody (FITC)

Binding Specificity: pSer299

Purpose: Phospho-A Raf (Ser299) Polyclonal Antibody, FITC Conjugated

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser299

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A Raf

Background:

Synonyms: A RAF phospho S299, p-A RAF phospho S299,A raf 1, A Raf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, ARaf proto oncogene serine/threonine protein kinase, ARAF1, Oncogene Araf1, Oncogene PKS2, PKS 2, PKS, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, ARAF_HUMAN.

Background: The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.

Gene ID: 369

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C