FEN1 antibody

Catalog No. ABIN7938132

This Rabbit Polyclonal antibody specifically detects FEN1 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for FEN1 antibody (ABIN7938132)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FEN1 Antibody

Purpose: FEN1 antibody (pAb)

Purification: Affinity Purified

Immunogen: This FEN1 antibody was raised against a peptide containing humanFlap endonuclease

Isotype: IgG

Application Details

Application Notes: WB: 1:250-1:1000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background in Western blot.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 30 % glycerol, 0.035 % sodium azide and 0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background: FEN1 - Flap endonuclease 1 (is recognized as a pivotal factor in DNA replication, long-patch excision repair, and telomere maintenance. FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.

Molecular Weight: 42 kDa

NCBI Accession: NP_004102

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA