Recombinant GADD34 antibody

Catalog No. ABIN7938135

The Rabbit Monoclonal anti-GADD34 antibody (Clone 4K3) (ABIN7938135) specifically detects GADD34 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant GADD34 antibody (ABIN7938135)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GADD34 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 4K3

Product Details anti-GADD34 Antibody

Purpose: GADD34 antibody (rAb), 100 μl (Clone 4K3)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the GADD34 fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:2000-1:16000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for GADD34

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: GADD34

Background:

Background_Plain: GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice.

Aliases: Growth-arrest- and DNA-damage-induced transcript 34, PPP1R15A

Molecular Weight: 73 kDa

Pathways: ER-Nucleus Signaling, Unfolded Protein Response