AIM2 antibody (AA 14-215) (PE)

Catalog No. ABIN7965393

The PE-conjugated Rabbit Polyclonal anti-AIM2 antibody (ABIN7965393) specifically detects AIM2 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AIM2 antibody (AA 14-215) (PE) (ABIN7965393)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIM2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-AIM2 Antibody (PE)

Binding Specificity: AA 14-215

Purpose: Anti-AIM2 Antibody PE Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: AIM2

Background:

Background: Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA  (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC  (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain  (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines  (IL-1b and IL-18).

Gene Full Name: absent in melanoma 2

Gene ID: 9447

UniProt: O14862

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome