AIM2 antibody (AA 136-354) (HRP)

Catalog No. ABIN7965402

The HRP-conjugated Rabbit Polyclonal anti-AIM2 antibody (ABIN7965402) specifically detects AIM2 in WB, ELISA and IHC. The antibody is reactive with Mouse samples.

Quick Overview for AIM2 antibody (AA 136-354) (HRP) (ABIN7965402)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIM2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-AIM2 Antibody (HRP)

Binding Specificity: AA 136-354

Purpose: Anti-Aim2 Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Aim2 recombinant protein (Position: Q136-E354).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: Aim2

Background:

Background: Interferon-inducible protein AIM2,also known asabsent in melanoma 2or simplyAIM2,is aproteinthat in humans is encoded by theAIM2gene. It is mapped to 1q22. AIM2 is a member of theIfi202/IFI16family. It plays a putative role in tumorigenic reversion and may control cell proliferation.Interferon-gammainduces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA(either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another proteinASC(or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains aCARD domain(caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation ofcaspase-1, an enzyme that processes proinflammatorycytokines(IL-1bandIL-18).

Gene Full Name: absent in melanoma 2

Gene ID: 383619

UniProt: Q91VJ1

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome