ATM antibody (N-Term) (Biotin)

Catalog No. ABIN7970502

This Rabbit Polyclonal antibody specifically detects ATM in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATM antibody (N-Term) (Biotin) (ABIN7970502)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ATM Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-Serine-protein kinase ATM ATM Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATM, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background:

Background: ATM (ataxia telangiectasia mutated),also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. Linkage analysis of ataxia-telangiectasia led to mapping of the ATM gene to chromosome 11q22.3. Using an antiserum developed to a peptide corresponding to the deduced amino acid sequence of ATM, the ATM protein is a single, high molecular weight protein predominantly confined to the nucleus of human fibroblasts, although it is present in both nuclear and microsomal fractions from human lymphoblast cells and peripheral blood lymphocytes. Overexpression of ATM cDNA in AT cells enhanced their survival after radiation exposure, decreased radiation-induced chromosome aberrations, reduced radioresistant DNA synthesis, and partially corrected defective cell cycle checkpoints and induction of stress-activated protein kinase. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors, in a telomere-independent manner. ATM functions ly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Gene Full Name: ATM serine/threonine kinase

Sequence Similarities: Belongs to the PI3/PI4-kinase family. ATM subfamily.

Gene ID: 472

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus