PUMA antibody (N-Term)

Catalog No. ABIN7972232

The Rabbit Polyclonal anti-PUMA antibody (ABIN7972232) specifically detects PUMA in WB. The antibody is reactive with Human samples.

Quick Overview for PUMA antibody (N-Term) (ABIN7972232)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-PUMA Antibody

Binding Specificity: N-Term

Purpose: Anti-PUMA/BBC3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PUMA/BBC3 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PUMA/BBC3, which shares 95.7% amino acid (aa) sequence identity with mouse and rat BBC3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: BBC3

Background:

Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Gene Full Name: BCL2 binding component 3

Molecular Weight: 18 kDa

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity