BARD1 antibody (AA 709-758) (Fluoro488)

Catalog No. ABIN7973149

The Rabbit Polyclonal anti-BARD1 antibody is suitable to detect BARD1 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for BARD1 antibody (AA 709-758) (Fluoro488) (ABIN7973149)

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BARD1 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-BARD1 Antibody (Fluoro488)

Binding Specificity: AA 709-758

Purpose: Anti-BARD1 Antibody Fluoro488 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BARD1 recombinant protein (Position: P709-A758).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for BARD1

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Alternative Name: BARD1

Background:

Background: BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: BRCA1 associated RING domain 1

Gene ID: 580

UniProt: Q99728

Pathways: DNA Damage Repair