FLT3 antibody (AA 62-295)

Catalog No. ABIN7991193

This Rabbit Polyclonal antibody specifically detects FLT3 in WB, ELISA and FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for FLT3 antibody (AA 62-295) (ABIN7991193)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-FLT3 Antibody

Binding Specificity: AA 62-295

Purpose: Anti-Flt3/CD135 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Flt3/CD135 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: Flt3

Background:

Background: Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.

Gene Full Name: FMS-like tyrosine kinase 3

Molecular Weight: 150 kDa

Gene ID: 14255

UniProt: Q00342

Pathways: RTK Signaling