GPBAR1 antibody (AA 76-310)

Catalog No. ABIN7992153

This Rabbit Polyclonal antibody specifically detects GPBAR1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GPBAR1 antibody (AA 76-310) (ABIN7992153)

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPBAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-GPBAR1 Antibody

Binding Specificity: AA 76-310

Purpose: Anti-GPCR TGR5/GPBAR1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GPCR TGR5/GPBAR1 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GPCR TGR5/GPBAR1 recombinant protein (Position: N76-S310).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GPBAR1

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Alternative Name: GPBAR1

Background:

Background: The G protein-coupled bile acid receptor 1 (GPBAR1) also known G-protein coupled receptor 19 (GPCR19), membrane-type receptor for bile acids (M-BAR) or TGR5 as is a protein that in humans is encoded by the GPBAR1 gene. This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.

Gene Full Name: G protein-coupled bile acid receptor 1

Molecular Weight: 45 kDa

Gene ID: 151306

UniProt: Q8TDU6

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound