Huntingtin antibody (AA 3079-3142) (Fluoro488)

Catalog No. ABIN7998424

The Rabbit Polyclonal anti-Huntingtin antibody is suitable to detect Huntingtin in samples from Human. It has been validated for FACS.

Quick Overview for Huntingtin antibody (AA 3079-3142) (Fluoro488) (ABIN7998424)

Target: Huntingtin (HTT)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-Huntingtin Antibody (Fluoro488)

Binding Specificity: AA 3079-3142

Purpose: Anti-Huntingtin/HTT Antibody Fluoro488 Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human HTT shares 93.8%,92.2% amino acid (aa) sequence identity with mouse,rat HTT,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Huntingtin/HTT recombinant protein (Position: R3079-C3142). Human HTT shares 93.8% and 92.2% amino acid (aa) sequence identity with mouse and rat HTT, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: HTT

Background:

Background: The huntingtin gene, also called HTT or HD(Huntington disease) gene, is the IT15(interesting transcript 15) gene which codes for a protein called the huntingtin protein. It is mapped to 4p16.3. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor(BDNF) at the transcription level, and this gene is required for normal development. The HTT protein is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. What's more, wildtype HTT may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

Gene Full Name: huntingtin

Gene ID: 3064

UniProt: P42858

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport