MCU antibody (AA 51-351)

Catalog No. ABIN8010651

The Rabbit Polyclonal anti-MCU antibody is suitable to detect MCU in samples from Human, Mouse, Rat and Monkey. It has been validated for ELISA, IHC, WB and FACS.

Quick Overview for MCU antibody (AA 51-351) (ABIN8010651)

Target: MCU (Mitochondrial Calcium Uniporter (MCU))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCU antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-MCU Antibody

Binding Specificity: AA 51-351

Purpose: Anti-MCU Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MCU Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCU recombinant protein (Position: V51-D351).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCU

Target: MCU (Mitochondrial Calcium Uniporter (MCU))

Alternative Name: MCU

Background:

Background: The mitochondrial calcium uniporter (MCU) is a transmembrane protein that allows the passage of calcium ions from a cell's cytosol into mitochondria. Its activity is regulated by MICU1 and MICU2, which together with the MCU make up the mitochondrial calcium uniporter complex. This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants.

Gene Full Name: mitochondrial calcium uniporter

Molecular Weight: 34 kDa

Gene ID: 90550

UniProt: Q8NE86

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis