MUS81 antibody (AA 6-545)

Catalog No. ABIN8012081

This Rabbit Polyclonal antibody specifically detects MUS81 in ELISA, WB and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for MUS81 antibody (AA 6-545) (ABIN8012081)

Target: MUS81 (MUS81 Endonuclease Homolog (MUS81))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUS81 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-MUS81 Antibody

Binding Specificity: AA 6-545

Purpose: Anti-MUS81 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MUS81 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MUS81 recombinant protein (Position: R6-C545).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MUS81

Target: MUS81 (MUS81 Endonuclease Homolog (MUS81))

Alternative Name: MUS81

Background:

Background: Crossover junction endonuclease MUS81 is an enzyme that in humans is encoded by the MUS81 gene. This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.

Gene Full Name: MUS81 structure-specific endonuclease subunit

Molecular Weight: 70 kDa

Gene ID: 80198

UniProt: Q96NY9

Pathways: DNA Damage Repair