NLRP3 antibody (AA 21-194)

Catalog No. ABIN8015878

The Rabbit Polyclonal anti-NLRP3 antibody (ABIN8015878) specifically detects NLRP3 in WB. The antibody is reactive with Human samples.

Quick Overview for NLRP3 antibody (AA 21-194) (ABIN8015878)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-NLRP3 Antibody

Binding Specificity: AA 21-194

Purpose: Anti-CIAS1/NALP3/NLRP3 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Characteristics: Anti-CIAS1/NALP3/NLRP3 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3

Background:

Background: NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Gene Full Name: NLR family pyrin domain containing 3

Sequence Similarities: Belongs to the NLRP family.

Molecular Weight: 118 kDa

Gene ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome