NUP214 antibody (AA 36-374)

Catalog No. ABIN8017309

The Rabbit Polyclonal anti-NUP214 antibody (ABIN8017309) specifically detects NUP214 in WB, IF, ICC, ELISA and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for NUP214 antibody (AA 36-374) (ABIN8017309)

Target: NUP214 (Nucleoporin 214kDa (NUP214))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP214 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-NUP214 Antibody

Binding Specificity: AA 36-374

Purpose: Anti-NUP214 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUP214 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUP214 recombinant protein (Position: K36-E374).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NUP214

Target: NUP214 (Nucleoporin 214kDa (NUP214))

Alternative Name: NUP214

Background:

Background: Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t (6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Gene Full Name: nucleoporin 214

Molecular Weight: 280 kDa

Gene ID: 8021

UniProt: P35658

Pathways: Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection