PLK1 antibody (AA 86-430) (Biotin)

Catalog No. ABIN8023722

The Mouse Monoclonal anti-PLK1 antibody is suitable to detect PLK1 in samples from Human and Monkey. It has been validated for WB, ELISA and IHC.

Quick Overview for PLK1 antibody (AA 86-430) (Biotin) (ABIN8023722)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 6D11

Product Details anti-PLK1 Antibody (Biotin)

Binding Specificity: AA 86-430

Purpose: Anti-PLK1 Antibody (monoclonal, 6D11) Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human PLK1 shares 95.4%,96.2% amino acid (aa) sequence identity with mouse,rat PLK1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background:

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

Gene Full Name: polo like kinase 1

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase