POLB antibody (AA 122-335)

Catalog No. ABIN8024097

The Rabbit Polyclonal anti-POLB antibody is suitable to detect POLB in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for POLB antibody (AA 122-335) (ABIN8024097)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-POLB Antibody

Binding Specificity: AA 122-335

Purpose: Anti-DNA Polymerase beta/POLB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human POLB shares 95.3%,94.9% amino acid (aa) sequence identity with mouse,rat POLB,respectively.

Characteristics: Anti-DNA Polymerase beta/POLB Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB

Background:

Background: Polymerase (DNA ed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Gene Full Name: DNA polymerase beta

Molecular Weight: 38 kDa

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair