SWAP70 antibody (AA 161-292) (Cy3)

Catalog No. ABIN8044010

This Rabbit Polyclonal antibody specifically detects SWAP70 in FACS. It exhibits reactivity toward Human.

Quick Overview for SWAP70 antibody (AA 161-292) (Cy3) (ABIN8044010)

Target: SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SWAP70 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-SWAP70 Antibody (Cy3)

Binding Specificity: AA 161-292

Purpose: Anti-SWAP70 Antibody Cy3 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SWAP70 recombinant protein (Position: H161-K292).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for SWAP70

Target: SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

Alternative Name: SWAP70

Background:

Background: SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators, in dendritic cells, it regulates the appearance of MHC II, and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96 % aa identity with mouse SWAP70.

Gene Full Name: switching B cell complex subunit SWAP70

Gene ID: 23075

UniProt: Q9UH65

Pathways: Production of Molecular Mediator of Immune Response