SWAP70 antibody (AA 161-292)

Catalog No. ABIN8044019

This Rabbit Polyclonal antibody specifically detects SWAP70 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for SWAP70 antibody (AA 161-292) (ABIN8044019)

Target: SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SWAP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-SWAP70 Antibody

Binding Specificity: AA 161-292

Purpose: Anti-SWAP70 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SWAP70 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SWAP70 recombinant protein (Position: H161-K292).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.5-1 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SWAP70

Target: SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

Alternative Name: SWAP70

Background:

Background: SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators, in dendritic cells, it regulates the appearance of MHC II, and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96 % aa identity with mouse SWAP70.

Gene Full Name: switching B cell complex subunit SWAP70

Molecular Weight: 69 kDa

Gene ID: 23075

UniProt: Q9UH65

Pathways: Production of Molecular Mediator of Immune Response