TDG antibody (AA 15-370) (Biotin)

Catalog No. ABIN8046533

This Rabbit Polyclonal antibody specifically detects TDG in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TDG antibody (AA 15-370) (Biotin) (ABIN8046533)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TDG Antibody (Biotin)

Binding Specificity: AA 15-370

Purpose: Anti-TDG Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TDG recombinant protein (Position: Q15-R370).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background:

Background: G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Gene Full Name: thymine DNA glycosylase

Gene ID: 6996

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding