WFS1 antibody (AA 61-313)

Catalog No. ABIN8054501

This Rabbit Polyclonal antibody specifically detects WFS1 in WB, IHC, ICC, ELISA, FACS and IF. It exhibits reactivity toward Human and Monkey.

Quick Overview for WFS1 antibody (AA 61-313) (ABIN8054501)

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WFS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-WFS1 Antibody

Binding Specificity: AA 61-313

Purpose: Anti-WFS1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-WFS1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human WFS1 recombinant protein (Position: A61-H313).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for WFS1

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Alternative Name: WFS1

Background:

Background: Wolframin is a protein that in humans is encoded by the WFS1 gene. This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene.

Gene Full Name: wolframin ER transmembrane glycoprotein

Molecular Weight: 100 kDa

Gene ID: 7466

UniProt: O76024

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome