TDP2 antibody (AA 201-300) (HRP)

Catalog No. ABIN755791

Product Details anti-TDP2 Antibody (HRP)

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Binding Specificity: AA 201-300

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDP2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ETS1 associated protein II/EAPII

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TDP2

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Alternative Name: ETS1 associated protein 2 (TDP2 Products)

Synonyms: AD022 antibody, EAP2 antibody, EAPII antibody, TTRAP antibody, dJ30M3.3 antibody, hTDP2 antibody, ad022 antibody, eap2 antibody, ttrap antibody, D13Ertd656e antibody, Ttrap antibody, tdp2 antibody, ttrapl antibody, tyrosyl-DNA phosphodiesterase 2 antibody, tyrosyl-DNA phosphodiesterase 2 S homeolog antibody, tyrosyl-DNA phosphodiesterase 2b antibody, TDP2 antibody, tdp2.S antibody, Tdp2 antibody, tdp2b antibody

Background:

Synonyms: EAP 2, EAP II, EAP2, EAPII, ETS 1 associated protein 2, ETS 1 associated protein II, ETS1 associated protein 2, ETS1-associated protein 2, ETS1-associated protein II, tdp2, TRAF and TNF receptor associated protein, TRAF and TNF receptor-associated protein, TTRAP, TYDP2_HUMAN, Tyr DNA phosphodiesterase 2, Tyr-DNA phosphodiesterase 2, Tyrosyl DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2, 5''-Tyr-DNA phosphodiesterase, 5''-tyrosyl-DNA phosphodiesterase, AD 022, AD022, MGC111021, MGC9099.

Background: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.