p21 antibody (pSer146) (Biotin)

Catalog No. ABIN756894

This anti-p21 antibody is a Rabbit Polyclonal antibody detecting p21 in ELISA, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for p21 antibody (pSer146) (Biotin) (ABIN756894)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-p21 Antibody (Biotin)

Binding Specificity: pSer146

Cross-Reactivity: Human

Predicted Reactivity: Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Ser146 [QT(p-S)MT]

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A

Background:

Synonyms: Activating Fragment 1, CAP20, Cation chloride cotransporter-interacting protein 1, CDK Interacting Protein 1, CDK-interacting protein 1, CDKI, CDKN 1, CDKN1, CDKN1A, CIP1, Cyclin Dependent Kinase Inhibitor 1A, Cyclin-dependent kinase inhibitor 1, Cyclin-dependent kinase inhibitor 1A P21, Cyclin-dependent kinase inhibitor 1A p21, Cip1, DNA Synthesis Inhibitor, MDA 6, MDA-6, MDA6, Melanoma Dferentiation Associated Protein 6, Melanoma dferentiation-associated protein 6, Melanoma dferentiation-associated protein, p21, P21 protein, p21CIP1, p21WAF, PIC1, SDI1, SLC12A9, WAF1, Wildtype p53 Activating Fragment 1, Wildtype p53-activated fragment 1.

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.

Gene ID: 1026

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy