DCLK1 antibody (AA 1-728)

Catalog No. ABIN8093134

This Rabbit Polyclonal antibody specifically detects DCLK1 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DCLK1 antibody (AA 1-728) (ABIN8093134)

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-DCLK1 Antibody

Binding Specificity: AA 1-728

Purpose: DCLK1 Antibody / Doublecortin-like kinase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human DCLK1 recombinant protein (amino acids M1-S728) was used as the immunogen for the Doublecortin-like kinase 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Doublecortin-like kinase 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Doublecortin-like kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCLK1

Background: Doublecortin-like kinase 1 antibody targets Doublecortin-like kinase 1, encoded by the DCLK1 gene. Doublecortin-like kinase 1 is a cytoplasmic serine-threonine kinase that combines microtubule-binding domains with a C-terminal kinase region, enabling coordination between cytoskeletal organization and intracellular signaling. Originally characterized in neuronal systems, DCLK1 is now recognized for its broader role in epithelial biology and cellular plasticity.

Functionally, Doublecortin-like kinase 1 contributes to regulation of cell morphology, migration, and differentiation by modulating microtubule stability and kinase-dependent signaling pathways. Through these combined activities, DCLK1 supports dynamic changes in cellular structure and behavior. A Doublecortin-like kinase 1 antibody supports studies focused on cytoskeletal signaling, cell fate regulation, and kinase-driven cellular processes.

DCLK1 expression is restricted to specific cell populations rather than being ubiquitous. In epithelial tissues, DCLK1 is frequently associated with rare cells exhibiting progenitor-like or regenerative characteristics. Its cytoplasmic localization and selective expression pattern have made DCLK1 a widely used marker in studies examining cellular heterogeneity and tissue renewal mechanisms.

From a disease-relevance perspective, Doublecortin-like kinase 1 has been extensively investigated in cancer research. Elevated DCLK1 expression has been linked to tumor initiation, progression, and resistance to therapy in multiple cancer types, particularly within the gastrointestinal tract. These findings have positioned DCLK1 as a biomarker of tumor-associated cell populations and a protein of interest for studies of cancer cell plasticity and signaling.

At the molecular level, Doublecortin-like kinase 1 contains conserved domains that regulate both microtubule interaction and kinase activity. Alternative splicing and post-translational regulation can influence its functional behavior and apparent migration in biochemical assays without altering the primary amino acid sequence. Doublecortin-like kinase 1 antibody reagents support research applications focused on cancer biology, progenitor cell regulation, and microtubule-associated kinase function, with NSJ Bioreagents providing reagents intended for research use.

UniProt: O15075