Recombinant HLA-DRA antibody (AA 1-200)

Catalog No. ABIN8093243

The Rabbit Monoclonal anti-HLA-DRA antibody is suitable to detect HLA-DRA in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant HLA-DRA antibody (AA 1-200) (ABIN8093243)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-470R

Product Details anti-HLA-DRA Antibody

Binding Specificity: AA 1-200

Purpose: HLA-DRA Antibody / Major histocompatibility complex class II DR alpha chain

Immunogen: A recombinant fragment (around amino acids 1-200) of human HLA-DR protein (exact sequence is proprietary) was used as the immunogen for the HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the HLA-DRA Antibody for IHC / HLA-DR alpha immunohistochemistry Antibody should be determined by the researcher.

2. This HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: HLA-DRA

Background: Human leukocyte antigen DR alpha (HLA-DRA) is a major histocompatibility complex class II protein encoded by the HLA-DRA gene and forms the alpha chain of the HLA-DR antigen complex involved in antigen presentation. This molecule pairs with the HLA-DR beta chain to form a heterodimeric receptor that presents processed peptide antigens to CD4-positive T lymphocytes, playing a critical role in adaptive immune responses. HLA-DRA is therefore a central component of the MHC class II antigen presentation pathway and is highly expressed in professional antigen-presenting cells including B lymphocytes, dendritic cells, macrophages, and certain activated epithelial and immune cells.

HLA-DRA Antibody Recombinant Rabbit MAb for IHC is used to detect expression of the HLA-DR alpha chain in formalin-fixed, paraffin-embedded tissues using immunohistochemistry. In normal tissues, HLA-DR alpha expression is typically observed in antigen-presenting cells within lymphoid organs and inflammatory microenvironments. Immunohistochemical staining often reveals membranous and cytoplasmic signal in B cells, macrophages, dendritic cells, and other immune cell populations involved in antigen processing and presentation. Detection of HLA-DRA by immunohistochemistry therefore provides valuable information about immune activation, antigen presentation, and inflammatory responses within tissue samples.

The HLA-DR antigen is widely used as a marker of immune activation and antigen-presenting cell identity in both normal and diseased tissues. Because the HLA-DR complex consists of alpha and beta chains encoded by separate genes, antibodies targeting the alpha chain such as HLA-DRA antibody reagents allow specific evaluation of this component of the MHC class II receptor. Expression of HLA-DR alpha is tightly regulated and can be induced by cytokines such as interferon-gamma during immune responses. As a result, HLA-DRA expression frequently increases in inflammatory conditions, infection, autoimmune disease, and tumor microenvironments where immune cell infiltration occurs.

In diagnostic and research pathology, HLA-DRA antibody staining is frequently used to evaluate immune cell populations and antigen presentation activity within tissues. The HLA-DR alpha chain is commonly detected in lymphoid tissues, sites of inflammation, and tumors with significant immune infiltration. Because HLA-DR expression reflects activation of antigen-presenting pathways, immunohistochemical analysis of HLA-DRA can help characterize immune microenvironments and assess interactions between immune cells and tumor cells. Clone MSVA-470R is a recombinant rabbit monoclonal antibody designed to recognize HLA-DRA and supports detection of the HLA-DR alpha chain in immunohistochemical staining applications.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response