PHLPP2 antibody (C-Term)

Catalog No. ABIN8107926

The Rabbit Polyclonal anti-PHLPP2 antibody (ABIN8107926) specifically detects PHLPP2 in WB. The antibody is reactive with Human samples.

Quick Overview for PHLPP2 antibody (C-Term) (ABIN8107926)

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHLPP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHLPP2 Antibody

Binding Specificity: C-Term

Purpose: PHLPP2 Antibody - C-terminal region

Sequence: PVPLSKVFSL EQDPEEAQRV KDQKAIITED NKVNGVTCCT RMLGCTYLYP

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PHLPP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42429-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHLPP2

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Alternative Name: PHLPP2

Background:

Background Information: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family, specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity.

Gene Name: PH domain and leucine rich repeat protein phosphatase 2

Alternative Symbols: PPM3B, PHLPPL

Protein Name: PH domain leucine-rich repeat-containing protein phosphatase 2

Molecular Weight: 138 kDa

Gene ID: 23035

NCBI Accession: NP_055835

UniProt: Q6ZVD8

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway