SIGLEC8 antibody (C-Term)

Catalog No. ABIN8111681

The Rabbit Polyclonal anti-SIGLEC8 antibody is suitable to detect SIGLEC8 in samples from Human. It has been validated for WB.

Quick Overview for SIGLEC8 antibody (C-Term) (ABIN8111681)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIGLEC8 Antibody

Binding Specificity: C-Term

Purpose: SIGLEC8 Antibody - C-terminal region

Sequence: ESWKDGNPLK KPPPAVAPSS GEEGELHYAT LSFHKVKPQD PQGQEATDSE

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human SIGL8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP74219-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background:

Background Information: Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM, MIM 603492) that mediates an association with SLAM-associated protein (SAP, MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Gene Name: sialic acid binding Ig like lectin 8

Alternative Symbols: SAF2, SIGLEC-8, SIGLEC8L

Protein Name: sialic acid-binding Ig-like lectin 8

Molecular Weight: 54kDa

Gene ID: 27181

NCBI Accession: NP_055257

UniProt: Q9NYZ4