Smooth Muscle Actin antibody

Catalog No. ABIN8119516

The Rabbit Monoclonal anti-Smooth Muscle Actin antibody (Clone AB1667) (ABIN8119516) specifically detects Smooth Muscle Actin in WB, IHC, IF, ELISA and IP. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Smooth Muscle Actin antibody (ABIN8119516)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB1667

Product Details anti-Smooth Muscle Actin Antibody

Purpose: α smooth muscle actin Rabbit mAb

Specificity: This antibody detects endogenous levels of α smooth muscle actin protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: alpha smooth muscle actin

Background:

Synonyms: ACTA2, ACTSA, ACTVS, GIG46, Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],

Gene Name: ACTA2

Protein Name: α smooth muscle actin

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development