Aurora A antibody

Catalog No. ABIN8119907

This Rabbit Monoclonal antibody specifically detects Aurora A in WB, ELISA, IHC, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Aurora A antibody (ABIN8119907)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1729

Product Details anti-Aurora A Antibody

Purpose: Aurora A Rabbit mAb

Specificity: This antibody detects endogenous levels of Aurora A protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora A

Background:

Synonyms: Aurora kinase A (EC 2. 7. 11. 1) (Aurora 2) (Aurora/IPL1-related kinase 1) (ARK-1) (Aurora-related kinase 1) (hARK1) (Breast tumor-amplified kinase) (Serine/threonine-protein kinase 15) (Serine/threonine-protein kinase 6) (Serine/threonine-protein kinase aurora-A)

Background: Aurora A (AURKA) Homo sapiens The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Gene Name: AURKA AIK AIRK1 ARK1 AURA AYK1 BTAK IAK1 STK15 STK6

Protein Name: Aurora A

Gene ID: 6790

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization