Caspase 4 antibody (Cleaved-Ala290)

Catalog No. ABIN8120234

This Rabbit Polyclonal antibody specifically detects Caspase 4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Caspase 4 antibody (Cleaved-Ala290) (ABIN8120234)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 4 Antibody

Binding Specificity: Cleaved-Ala290

Purpose: Caspase 4 (p10, Cleaved-Ala290) Antibody

Specificity: This antibody detects endogenous levels of Human Caspase 4 (p10, Cleaved-Ala290, protein was cleaved amino acid sequence between 289-290)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human Caspase 4 (p10, Cleaved-Ala290)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase 4

Background:

Synonyms: Caspase-4 (CASP-4, EC 3.4.22.57, ICE(rel)-II, Protease ICH-2, Protease TX) [Cleaved into: Caspase-4 subunit 1, Caspase-4 subunit 2]

Background: catalytic activity:Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.,PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit.,tissue specificity:Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.,

Gene Name: CASP4 ICH2

Protein Name: Caspase4

Gene ID: 837

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity