MIB2 antibody

Catalog No. ABIN8121115

The Rabbit Polyclonal anti-MIB2 antibody (ABIN8121115) specifically detects MIB2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MIB2 antibody (ABIN8121115)

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MIB2 Antibody

Purpose: MIB2 Antibody

Specificity: MIB2 Polyclonal Antibody detects endogenous levels of protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MIB2

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Alternative Name: MIB2

Background:

Background: function:E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.,induction:Down-regulated in many primary skin melanomas. Treatment with a demethylating agent, 5'-aza-2-deoxycytidine, restores expression, suggesting that down-regulation is the result of methylation of the gene.,pathway:Protein modification, protein ubiquitination.,PTM:Ubiquitinated. Possibly via autoubiquitination.,similarity:Contains 1 ZZ-type zinc finger.,similarity:Contains 2 MIB/HERC2 domains.,similarity:Contains 2 RING-type zinc fingers.,similarity:Contains 9 ANK repeats.,subcellular location:Colocalizes with endosomal compartments.,subunit:Interacts with actin monomer.,tissue specificity:Expressed in skeletal muscle, and to a lesser extent in heart, brain and kidney.,

Gene Name: MIB2 SKD ZZANK1

Protein Name: E3 ubiquitin-protein ligase MIB2 (EC 6.3.2.-) (Mind bomb homolog 2) (Novel zinc finger protein) (Novelzin) (Putative NF-kappa-B-activating protein 002N) (Skeletrophin) (Zinc finger ZZ type with ankyrin repeat domain protein 1)

Gene ID: 142678

UniProt: Q96AX9

Pathways: Notch Signaling